预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中肾上腺素能a1A受体(ADRA1A)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中ADRA1A水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ADRA1A、生物素化的抗大鼠ADRA1A抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ADRA1A呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat Alpha-1-adrenergic receptor,ADRA1A concentrations in cell culture supernates, serum, and plasm
Introduction
The alpha 1c-Adrenergic Receptor, also known as alpha 1a-adrenoceptor, is activated by epinephrine and norepinephrine. This receptor mediates calcium release upon ligand binding. The alpha 1-c adrenoceptor is one of three subtypes of alpha 1-adrenergic receptor (alpha 1 c/a, alpha 1b, and alpha 1d). The alpha 1c receptor couples to phospholipase C via a GTP-binding protein insensitive to pertussis toxin. Three isoforms are generated by alternative splicing. However, the corresponding isoforms of the protein have similar ligand-binding properties, effector coupling, and tissue distribution. Expression of this receptor has been reported in prostate, liver, kidney, heart, brain, spinal cord, bladder, and various blood vessels. ESTs have been isolated from liver and testis libraries. Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ADRA1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRA1A present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for ADRA1A is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRA1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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