预期应用
ELISA法定量测定猪血清、血浆、细胞培养物上清或其它相关液体中BAX含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中BAX水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入BAX抗原、生物素化的抗猪BAX抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的BAX呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of porcine BAX concentrations in cell culture supernates, serum, and plasma.
Introduction
Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade. Protein levels of Bax and other Bcl-2 family members are altered in various human malignancies, with changes in expression levels implicated in diseases involving apoptosis such as cancer, autoimmune diseases and neurodegenerative disorders.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BAX present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for BAX is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of BAX bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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