预期应用
ELISA法定量测定人血清、血浆或其它相关液体中DR-70含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中DR-70水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入DR-70抗原、生物素化的抗人DR-70抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的DR-70呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human DR-70 concentrations in serum and plasma.
Introduction
The DR-70 is an antigen that target the proteinase cathepsin B, which is one kind of fibrinogen degradation protein (FDP) as plasiminogen activator that were normally secreted by tumor cell for fibrinose lysis. The general structure of cathepsin B is found at PDB protein database. DR-70 is marketed as a lung cancer tumor-marker.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for DR-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DR-70 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for DR-70 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of DR-70 bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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