预期应用
ELISA法定量测定猪血浆、或其它相关液体中Ghrelin含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Ghrelin水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ghrelin抗原、生物素化的抗猪Ghrelin抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ghrelin呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of porcine Ghrelin concentrations in plasma.
Introduction
Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a small peptide consisting of 28-amino acids by Dr. Kenji Kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. Ghrelin has been implicated in regulation of hunger and long-term weight gain/loss. Ghrelin is the endogenous ligand for the growth hormone secretagogue (GHS) receptor and has potent growth hormone-releasing activity. This peptide may constitute a new regulatory mechanism for GH-release. It is conceivable that Ghrelin may also have other functions in some tissues in addition to pituitary, because the GHS receptor is expressed in heart, lung, pancreas, intestine, and adipose tissue.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Ghrelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ghrelin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Ghrelin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ghrelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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