本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IMA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入IMA抗原、生物素化的抗人IMA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IMA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Ischemia Modified Albumin,IMA concentrations in cell culture supernates, serum, and plasma.
Introduction
Ischemia-modified albumin (IMA) is a novel highly sensitive, fairly specific biomarker of myocardial ischemia. IMA can be detected via the albumin cobalt binding (ACB) test, a limited available FDA approved assay. Myocardial ischemia alters the N-terminus of albumin reducing the ability of cobalt to bind to albumin. IMA measures ischemia in the blood vessels and thus returns results in minutes rather than traditional markers of necrosis that take hours. ACB has low specificity therefore generating high number of false positives and must be used in conjunction with typical acute approaches such as ECG and physical exam. Additional studies are required.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for IMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IMA present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for IMA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
