预期应用
ELISA法定量测定兔血清、血浆或其它相关液体中hs-CRP含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中hs-CRP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入hs-CRP抗原、生物素化的抗兔hs-CRP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的hs-CRP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
特异性
本试剂盒可同时检测重组或天然的兔hs-CRP，且与其它相关蛋白无交叉反应。
检测范围：
1.56 ng/mL -100 ng/mL
Intended use
This immunoassay kit allows for the specific measurement of rabbit Hs-CRP concentrations in serum and plasma.
Introduction
C-reactive Protein (CRP) is mainly formed in hepatocytes. The synthesis rate is determined by the influence of cytokines involved in inflammatory processes. The biological half-life is estimated to be 13-16 hours. A connection between inflammatory reactions and cardiovascular diseases such as arteriosclerosis and latent or chronically persisting infections has been described in recent studies. CRP belongs to the acute phase proteins and will be released within 24 hours after inflammatory process started (fever, pneumonia, myocardial infarction, etc.).
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Hs-CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hs-CRP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Hs-CRP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hs-CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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