本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PCX水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PCX抗原、生物素化的抗人PCX抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PCX呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Podocalyxin concentrations in urine.
Introduction
Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Podocalyxin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Podocalyxin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Podocalyxin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Podocalyxin bound in the initial step. The color development is stopped and the intensity of the color is measured.
