本试剂盒应用双抗体夹心酶标免疫分析法测定标本中α葡萄糖苷酶水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入α葡萄糖苷酶抗原、生物素化的抗小鼠α葡萄糖苷酶抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的α葡萄糖苷酶呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of total Mouseα-Glucosidase concentrations in cell culture supernates, serum and plasma.
Introduction
Glucosidases are glycoside hydrolase enzymes categorized under the EC number 3.2.1.
α-glucosidases are enzymes involved in breaking down complex carbohydrates such as starch and glycogen
Alpha-glucosidase (EC 3.2.1.20 ) (alternative names: maltase-glucoamylase, MGAM; acid maltase; glucoinvertase; glucosidosucrase; lysosomal α-glucosidase; maltase) is found in the mammalian intestine, and has similar enzymatic activity to γ-amylase.They are targeted by alpha-glucosidase inhibitors such as acarbose and miglitol to control diabetes mellitus type 2. They catalyze the cleavage of individual glucosyl residues from various glycoconjugates including alpha- or beta-linked polymers of glucose.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for α-glucosidases has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any α-glucosidases present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for α-glucosidases is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of α-glucosidases bound in the initial step. The color development is stopped and the intensity of the color is measured.
