本试剂盒应用双抗体夹心酶标免疫分析法测定标本中甘丙素水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入甘丙素、生物素化的抗大鼠甘丙素抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的甘丙素呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat galanin,GAL concentrations in cell culture supernates, serum, and plasma.
Introduction
Galanin is a 30 amino acid neuropeptide which is cleaved from preprogalanin. It is involved in a number of physiological processes such as regulation of food intake, metabolism and reproduction, regulation of neurotransmitter and hormone release, nociception, intestinal contraction and secretion, and more recently in nervous system development and response to injury. This wide diversity of action is mediated by several galanin receptor subtypes.
Galanin is predominantly an inhibitory, hyperpolarizing neuropeptide and as such inhibits neurotransmitter release. The Galanin receptor is often co-localized with classical neurotransmitters such as acetylcholine, dopamine, serotonin and norepinephrine and also with other neuromodulators such as Neuropeptide Y, Substance P and Vasoactive Intestinal Peptide.
The galanin receptor is a G protein-coupled receptor, or metabotropic receptor which binds galanin.So far three subtypes are known to exist: GAL-R1, GAL-R2 and GAL-R3. The specific function of each subtype remains to be fully elucidated, although great progress is currently being made in this respect with the generation of receptor subtype-specific knockout mice.
Galanin receptors can be found throughout the peripheral and central nervous systems and the endocrine system.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAL present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for GAL is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
