本试剂盒应用双抗体夹心酶标免疫分析法测定标本中吲哚乙酸水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入吲哚乙酸、生物素化的抗植物吲哚乙酸抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的吲哚乙酸呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Plant Indole-3-acetic acid ,IAA concentrations in Plant tissue or cell culture supernates.
Introduction
Indole-3-acetic acid, also known as IAA, is a member of the group of phytohormones called auxins. IAA is generally considered to be the most important native auxin.
It is produced in cells in the apex (bud) and young leaves of a plant. Plant cells synthesize IAA from tryptophan. It has many different effects, as all auxins do, such as inducing cell elongation and cell division with all subsequent results for plant growth and development. There are less expensive and metabolically stable synthetic auxin analogs on the market for use in horticulture, such as indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA).
Studies of IAA in the 1940s led to the development of the phenoxy herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). Like IBA and NAA, 2,4-D and 2,4,5-T are metabolically and environmentally more stable analogs of IAA. However, when sprayed on broad-leaf dicot plants, they induce rapid, uncontrolled growth, eventually killing them. IAA can be produced by the reaction of indole with potassium glycolate at 250 C, but this compound is chemically unstable in particular when exposed to light or changing temperature.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IAA present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for IAA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
