本试剂盒应用双抗体夹心酶标免疫分析法测定标本中赤霉素水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入赤霉素、生物素化的抗植物赤霉素抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的赤霉素呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Plant Gibberellic Acid,GA concentrations in Plant tissue or cell culture supernates.
Introduction
Gibberellic acid (also called Gibberellin A3, GA, and (GA3) is a hormone found in plants. Its chemical formula is C19H22O6. When purified, it is a white-to-pale-yellow crystalline powder, soluble in ethanol and slightly soluble in water.
Gibberellic acid is a simple gibberellin, promoting growth and elongation of cells. It affects decomposition of plants and helps plants grow if used in small amounts, but eventually plants develop tolerance for it. Gibberellic acid stimulates the cells of germinating seeds to produce mRNA molecules that code for hydrolytic enzymes. Gibberellic acid is a very potent hormone whose natural occurrence in plants controls their development. Since GA regulates growth, applications of very low concentrations can have a profound effect while too much will have the opposite effect. It is usually used in concentrations between 0.01-10 mg/L.
Gibberellins have a number of effects on plant development. They can stimulate rapid stem growth,induce mitotic division in the leaves of some plants,increase seed germination rate.
Gibberellic acid is sometimes used in laboratory and greenhouse settings to trigger germination in seeds that would otherwise remain dormant. It is also widely used in the grape-growing industry as a hormone to induce the production of larger bundles and bigger grapes, especially Thompson seedless grapes, and in the Okanagan it is used in the cherry industry as a growth regulator.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GA present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for GA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GA bound in the initial step. The color development is stopped and the intensity of the color is measured.
