本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CYPA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CYPA抗原、生物素化的抗人CYPA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CYPA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human CYPA concentrations in cell culture supernates, serum, and plasma.
Introduction
Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins. Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin, making it a useful inhibitor for studying calcineurin activity.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CYPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYPA present is bound by the immobilized antibody. Anenzyme-linked monoclonal antibody specific for CYPA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
