本试剂盒应用双抗体夹心酶标免疫分析法测定标本中SGLT1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入SGLT1抗原、生物素化的抗猪SGLT1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的SGLT1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of porcine SGLT1 concentrations in serum and plasma.
Introduction
Sodium-glucose transport proteins are a family of
glucose transporter found in the intestinal
mucosa of the
small intestine (SGLT1) and the
proximal tubule of the
nephron (SGLT2 and SGLT1). SGLT1 and SGLT2 are members of the SLC5A gene family. SGLT1 located in the S3 segment of the proximal tubule. It has a 2Na+:1Glucose co-transport ratio and is responsible for 2% of glucose reabsorption
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for SGLT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SGLT1 present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for SGLT1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGLT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
