预期应用
ELISA法定量测定人血清、血浆或其它相关生物液体中MSTN含量。
实验原理
用纯化的MSTN抗体包被微孔板，制成固相载体，往微孔中依次加入标本或标准品、生物素化的MSTN抗体、HRP标记的亲和素，经过彻底洗涤后用底物(TMB)显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的MSTN呈正相关。用酶标仪在450nm波长下测定吸光度（ 值），计算样品浓度。
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human MSTN concentrations in serum, plasma and other biological fluids.
Introduction
Myostatin (formerly known as Growth differentiation factor 8) is a growth factor that limits muscle tissue growth, i.e. higher concentrations of myostatin in the body may cause the individual to have less developed muscles. The myostatin protein is produced primarily in skeletal muscle cells, circulates in the blood and acts on muscle tissue, apparently by slowing down the development of muscle stem cells. The precise mechanism remains unknown. Its functions in non-mammalian vertebrates appear to be somewhat conserved as muscle-specific actions have been demonstrated in birds. However, it is produced in many different fish tissues, suggesting that it may regulate more than just muscle mass in these vertebrates.
Myostatin is a member of the TGF beta superfamily of proteins. Human Myostatin consists of two identical subunits, each consisting of 110 amino acid residues. Its total molecular weight is 25.0 kDa. It can be produced in genetically engineered E. coli or eukaryotic cells and the recombinant protein from both sources is commercially available. However, due to the unique manner by which the mature protein is processed, there is considerable doubt as to the effectiveness of myostatin generated in E. coli.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MSTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of MSTN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
