预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中胶质细胞系来源的神经营养因子(GDNF)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GDNF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GDNF、生物素化的抗大鼠GDNF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GDNF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat glial cell line-derived neurotrophic factor,GDNF concentrations in cell culture supernates, serum, and plasma.
Introduction
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a recently discoved neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain
dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-superfamily.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDNF present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for GDNF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
| 
