预期应用
ELISA法定量测定猪血清、血浆、细胞培养物上清或其它相关液体中脑钠素（BNP）含量。
概述
脑钠素(BNP)是继心钠素(ANP)后发现的另一种心脏利钠肽，主要是由心室分泌，与ANP具有相似的扩张血管、利尿、利钠、抑制醛固酮分泌及交感神经活性等广泛的心血管作用，能较敏感地反映心功能状态。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中BNP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入BNP抗原、生物素化的抗猪BNP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的BNP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Porcine Brain natriuretic peptide ,BNP concentrations in cell culture supernates, serum, and plasma.
Introduction
Brain natriuretic peptide (BNP, also known as B-type natriuretic peptide or "GC-B") is a natriuretic hormone that is similar to ANP. It is a 32-amino-acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes (heart muscles cells).
Cardiac natriuretic hormones (CNHs) are a family of related peptides, including atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and other peptides derived from the N-terminal portion of the proANP and proBNP peptide chains. Brain natriuretic peptide (also known as B-type natriuretic peptide or "GC-B") is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes (heart muscles cells) in the ventricles. At the time of release, a co-secreted 76 amino acid N-terminal fragment (NT-proBNP) is also released with BNP. BNP binds to and activates NPRA in a similar fashion to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP. Both ANP and BNP have limited ability to bind and activate NPRB.
Brain natriuretic peptide was originally identified in extracts of porcine brain, but in humans it is produced mainly in the cardiac ventricles.
Physiologic actions of BNP and ANP include decrease in systemic vascular resistance and central venous pressure as well as an increase in natriuresis. Thus, the resulting effect of these peptides is a decrease in cardiac output and a decrease in blood volume. BNP is a valuable marker in heart failure and its therapy, for example cardiac resynchronization therapy.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BNP present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for BNP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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