预期应用
ELISA法定量测定小鼠血清、血浆、细胞培养物上清或其它相关液体中CRP含量。
概述
C-反应蛋白（CRP，C-Reactive Protein）是一种由肝脏生成出来的特殊蛋白，因为对肺炎球菌的C 多糖体会有反应，所以叫做C 反应蛋白。C-活性蛋白（CRP）是炎症和感染等症状的一个临床标记，但它也与受损的细胞结合，激发其补体（即参与免疫反应的血清蛋白复合物）。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CRP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CRP抗原、生物素化的抗小鼠CRP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CRP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Mouse C-Reactive Protein (CRP) concentrations in cell culture supernates, serum, and plasma.
Introduction
C-Reactive Protein (CRP) is the prototypical acute phase protein in humans and is an important mediator of immune host defense. The CRP gene consists of two exons and one intron. It is synthesized as a 206 amino acid polypeptide, and it is secreted as a ~23 kDa, non-glycosylated monomer that non-covalently associates to form the homopentameric ring structure characteristic of the pentraxin family. It is mainly produced by the liver in response to pro-inflammatory conditions. Normal baseline levels of circulating CRP are low, but may increase 10,000-fold within hours of inflammation induced by infection or injury. In contrast to human, mouse CRP is expressed at a very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. Like other pentraxins, CRP exhibits Ca2+- dependent binding to ligands. Phosphocholine, a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP also binds the membrane of injured cells as well as nuclear components of necrotic and apoptotic cells Upon binding with ligands, CRP is recognized by C1q and initiates activation of the complement cascade. Ligand-bound CRP also binds to Fcγ receptors and activates phagocytic responses. Although the physiological role of CRP is unclear, these activities suggest that CRP functions as an anti-bacterial protein that aids in the clearance of damaged and apoptotic cells.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CRP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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