预期应用
ELISA法定量测定牛血清、血浆、细胞培养物上清或其它相关液体中 CRP含量。
概述
C-反应蛋白（CRP，C-Reactive Protein）是一种由肝脏生成出来的特殊蛋白，因为对肺炎球菌的C 多糖体会有反应，所以叫做C 反应蛋白。C-活性蛋白（CRP）是炎症和感染等症状的一个临床标记，但它也与受损的细胞结合，激发其补体（即参与免疫反应的血清蛋白复合物）。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CRP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CRP抗原、生物素化的抗牛CRP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CRP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of bovine C-Reactive Protein (CRP) concentrations in cell culture supernates, serum, and plasma.
Introduction
C-reactive protein (CRP) has been regarded as an acute phase reactant in serum. It consists of five single subunits, which noncovalently linked and assembled, as a cyclic pentamer with a mol. Wt. Range of 110-140 kDa. CRP has been found to be increased in serum of patients with a wide variety of diseases including infections by gram-positive and gram-negative bacteria, acute phase of rheumatoid arthritis, abdominal abscesses, inflammation of bile ducts, myocardial infarction, and malignant tumors. CRP may be found in patients with Guillain-Barre syndrome and multiple sclerosis, certain viral infections, tuberculosis, acute infectious hepatitis, many other necrotic and inflammatory diseases, burned patients, and after surgical trauma. Although the detection of elevated levels of CRP in the serum is not specific for any particular disease, it is useful indicator of inflammatory processes. CRP levels rise in serum within hours of the onset of inflammation, reach a peak during the acute stage and decrease with resolution of inflammation trauma. The detection of CRP is a more reliable and sensitive indicator of the inflammatory process than the erythrocyte sedimentation rate, which may also be influenced by physiological changes not associated with an inflammation process.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CRP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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