心肌钙蛋白Ⅰ（Cardiac Troponin Ⅰ，CTnⅠ）作为心肌损伤的新的标志物，由于其高度的心肌特异性，高度敏感性及其较长的诊断窗口期，CTnⅠ已被广泛应用于临床。CTnⅠ在正常人血中含量极少，不易检测到。而在心肌损伤后4～8小时在外周血中逐渐增高，其浓度最高出现在12～24小时左右。在心肌损伤后7～10天外周血中仍可检测到CTnⅠ。
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中cTn-Ⅰ水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入cTn-Ⅰ抗原、生物素化的抗小鼠cTn-Ⅰ抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的cTn-Ⅰ呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of mouse cardiac troponinⅠ, cTn-Ⅰ concentrations in cell culture supernates, serum and plasma.
Introduction
Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.
Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A antibody specific for CTn-Ⅰhas been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTn-Ⅰpresent is bound by the immobilized antibody. An enzyme-linked antibody specific for CTn-Ⅰis added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTn-Ⅰbound in the initial step. The color development is stopped and the intensity of the color is measured.
