预期应用
ELISA法定量测定人血清、血浆或其它相关液体中cTnI含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中cTnI水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入cTnI抗原、生物素化的抗人cTnI抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的cTnI呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human cTnI concentrations in serum and plasma.
Introduction
Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located
periodically along the thin filament of the muscle and consists of three distinct proteins: troponin I, troponin C, and troponin T. Likewise, the troponin I subunit exists in three separate isoforms; two in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle. The cardiac isoform (cTnI) is about 40% dissimilar, has a molecular weight of 22,500 daltons, Cardiac troponin I (cTnI) has been useful in the differential diagnosis of patients presenting to Emergency Departments (ED) with chest pain. 18-20 Myocardial infarction is diagnosed when blood levels of sensitive and specific biomarkers, such as cardiac troponin, the MB isoenzyme of creatine kinase (CK-MB), and myoglobin, are increased in a clinical setting of acute ischemia. The most recently described and preferred biomarker for myocardial damage is cardiac troponin (I or T). The cardiac troponins exhibit myocardial tissue specificity and high sensitivity. The level of cTnI remains elevated for a much longer period of time (6-10 days), thus providing for a longer window of detection of cardiac injury. Normal levels of cTn I in the blood are very low. After the onset of an AMI, cTnI levels increase substantially and are measurable in serum within 4 to 6 hours, with peak concentrations reached in approximately 12 to 24 hours after infarction. The cTnI Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of cardiac-specific troponin I.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for cTnI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTnI present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for cTnI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTnI bound in the initial step. The color development is stopped and the intensity of the color is measured.
|
